Figure 2:

In vitro methods for determining RBP targets. (A) SELEX consists of several rounds of binding and amplification of RNA molecules. SEQRS modifies traditional SELEX by sequencing the bound pool of RNA at each round. (B) RNAcompete queries a designed RNA pool under competitive conditions and assays the bound RNAs using a microarray. (C) RNA Bind-n-Seq assays RNA binding by incubating RNA and various amounts of protein and sequencing the bound RNAs.