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. 2014 Nov 19;105(2):160–170. doi: 10.1093/cvr/cvu243

Figure 7.

Figure 7

Loss of PHLPP1 in cardiomyocytes increases endothelial cell tube formation. NRVMs were transfected with scrambled control or PHLPP1 siRNA. Following 48 h, (A) BAECs were co-cultured with the NRVMs in the presence of serum (5%) and bFGF (3 ng/mL) for 6 h. Bright field images were taken at ×10 magnification. Quantification of tubes per field was determined. (The graph represents n = 3 independent experiments, six fields per experiment; *P<0.05 compared with siControl.) (B) Isolation of RNA from NRVMs with scrambled control or PHLPP1 siRNA with or without serum (5%) and bFGF (3 ng/mL) for 6 h (no BAEC). The relative mRNA fold changes in VEGFa were determined compared with siControl (no treatment). [The graph represents n = 6 independent experiments; **P<0.01 compared with siControl (treated).]