Fig. 1.

Dietary effects on Lgr5+ cells in the small intestine. (A) Lgr5 ^GFP+ mice, four per dietary group were fed AIN76A, NWD1 or NWD2 diets from weaning for 3 months. Single suspensions of epithelial cells were prepared from isolated intestinal crypts and percent of these cells exhibiting green fluorescence determined by FACS analysis (mean ± std; *P < 0.03, analysis of variance); (B) Lgr5 ^GFP+ , Rosa26 ^RFP+ mice, nine per dietary group were fed diets from weaning for 3 months, then given a single injection of tamoxifen and three mice in each group killed at 1, 3 or 5 days later; (C) Lgr5 ^GFP+ , Rosa26 ^RFP+ mice were fed diets from weaning for 1 year, then given a single injection of tamoxifen and killed 3 days later. In (B) and (C), frozen sections of the intestine were stained with DAPI.