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. 2015 Jan 9;7(1):500–516. doi: 10.3390/nu7010500

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© 2015 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).

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β-cell Proliferation Rates. Pancreas tissues were immunostained for phosphorylated-Histone H3 (pHH3; green), insulin (β-cells; red), and nuclear DNA (DAPI; blue). Representative micrographs are presented for control-sham (A); control-AD (B); IUGR-sham (C); and IUGR-AD (D). The mean (±SEM) percentages of pHH3 positive β-cells are graphed for treatment groups labeled on the x-axis (E). Error bars represent the SEM and different superscripts denote statistically significant differences among treatment groups (p < 0.05).