Figure 3. Prenylation-mediated anchoring of Rap1 to Golgi-network is required for the proprotein convertase activation.

(a, b) Eph4/Rheo-Sipa1 cells were cultured in the absence or presence of RSL1 (a) or GGTI (b) for 3 days, and the intracellular proprotein convertase activity was assessed with or without EDTA. (c) Eph/Rheo-Sipa1 cells were cultured in the absence (left) or presence (right) of 4 μM GGTI for 24 h and multi-color immunostained with the indicated antibodies.