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. 2014 Nov 13;91(1):117–123. doi: 10.1111/php.12354

Figure 3.

Figure 3

Identification of full length and truncated splice variant of OPN3 in HEMs and KERs. (A) RT-PCR using HEM or KER RNA and primers based on exon 1 and exon 4 of OPN3 amplified two bands that corresponded to exons 1–4 (e1-e2-e3-e4, upper band) and to a splice variant missing exon 2 (e1-e3-e4, lower band) (Fig. S2A). (B) The cDNA amplified in the upper band in (A) corresponds to a fragment of the coding sequence of OPN3 that encompasses all four exons and results in a 403 amino acids peptide containing the retinal binding residue K299. (C) The cDNA amplified in the lower band in (A) corresponds to exons 1, 3 and 4 of OPN4, coding for a 143 amino acid peptide that does not contain the K299 residue (Fig. S2B).