FIG. 3.

Characterization of the WAT of PPARγ2 knockout mice. A: Representative histological appearance of hematoxylin-eosin–stained sections from epididymal WAT, from male wild-type and PPARγ2 knockout mice fed a normal diet (n = 7), and from epididymal and subcutaneous WAT depots from mice fed a 16-week HFD (n = 5). B: Area of epididymal and subcutaneous WAT adipocytes (200 random adipocytes) from wild-type and PPARγ2 knockout mice fed a normal diet and an HFD (n = 5). **P < 0.01 (Kruskal-Wallis test). C: Time course of WAT preadipocyte differentiation from wild-type and PPARγ2 knockout mice. Cells were observed by light microscopy after 3, 4, and 6 days of differentiation with standard differentiation-induction medium (representative experiment of three). D: Oil Red O staining of WAT adipocytes after 8 days of differentiation from wild-type and PPARγ2 knockout mice. E: 8 days of differentiation of WAT preadipocytes from wild-type and PPARγ2 knockout mice with and without BRL treatment.