Figure 4.
HGAL relocalizes to BCR interaction membrane regions after binding to anti-IgM. U2932 cells stably expressing HGAL-GFP were pretreated with dimethylsulfoxide (control; “untreated”) or Syk inhibitor (20 nM, BAY61-3606; “Syk-inhibited”) for 30 minutes and then seeded on 8-well slides (ibidi, Verona, WI) coated with phycoerythrin-conjugated anti-human IgM F(ab′)2. Cells were fixed at 0 and 30 minutes with 3.5% paraformaldehyde and used for images as described in the “Materials and methods” section. No attachment with membrane spreading was observed on non–anti-human anti-IgM F(ab′)2-coated slides. Ig, immumoglobulin.