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. 2014 Dec 10;125(4):687–696. doi: 10.1182/blood-2014-07-588590

Figure 4.

Figure 4

P-/L-selectin mediated rolling. Cell-adhesion studies were performed identically to Figure 3 only the substrates were composed of either P-selectin–expressing CHO-P cells (A-B,E-F), or recombinant L-selectin (C-D,G-H). Top panels present rolling and adherent cell density data, whereas bottom panels present cumulative rolling velocity distribution. A, E and C, G present data for HL-60 and its derivatives, whereas the remaining panels present murine neutrophil data. All statistical symbols are identical to Figure 3, except that WT+2PH1 is significantly different from ST3Gal-4−/− in panel B. Silencing ST3Gal-4 reduced HL-60 rolling on both P- and L-selectin by >90%. ST3Gal-4−/− mouse neutrophils rolled well on P-selectin unlike the ST3Gal-4− HL-60s. mAbs G1 and Dreg56 are anti-P- and L-selectin blocking mAbs.