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. 2014 Dec 31;33(1):113. doi: 10.1186/s13046-014-0113-6

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© Zhang et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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TGFBR2 was a direct target gene of miR-301a. (A) qRT-PCR showed that neither miR-301a mimics nor anti-miR-301a changed the mRNA level of TGFBR2. (B) anti-miR-301a in SW620 cells suppressed, while miR-301a mimics in SW480 cells increased the protein expression of TGFBR2. (C) Immunohistochemical staining of TGFBR2 in tumor sections. Expression of TGFBR2 was evaluated in tumors formed by SW620/LV-anti-miR-301a cells. (D) Schematic picture of two miR-301a binding sites and the corresponding mutants on TGFBR2 3’-UTR. (E) Luciferase activity assay identified the binding sites of miR-301a. Data were representedas means ± SD from 3 independent experiments. Magnification, ×200. Scale bars, 100 μm. *, P < 0.05; **, P < 0.01.