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. Author manuscript; available in PMC: 2015 Dec 1.
Published in final edited form as: Free Radic Biol Med. 2014 Sep 16;77:183–194. doi: 10.1016/j.freeradbiomed.2014.08.030

Fig. 8. Effects of HIF-1α inhibition in binge alcohol-exposed mice.

Fig. 8

(A-D) Immunoblot analyses of HIF-1α, BNIP3 (A), iNOS, 3-NT (B), CYP2E1 (C). Densitometric analysis was performed for each target protein and normalized to GAPDH or β-actin. Each number on top of the panels represents the average density of two lanes compared to the WT control (100). Representative TUNEL staining for apoptotic hepatocytes (arrows) (D) in WT mice exposed to binge alcohol for 6 h(i.e., 30 h from the first ethanol dose) in the absence or presence of PX-478 pretreatment. Data expressed as means ± SE(*p < 0.05 and **p< 0.01).