Table 2.
Antigen | Species | Working | dilution | Buffer* | Source |
---|---|---|---|---|---|
ADH | Rabbit | 1:5000 | 3% milk | Cell Signaling | |
CYP2E1 | Rabbit | 1:10000 | 3% milk | Abcam | |
Catalase | Rabbit | 1:2000 | 3% milk | Abcam | |
ALDH2 | Goat | 1:3000 | 3% milk | Santa Cruz Biotechnology | |
ATP-Synthase | Rabbit | 1:3000 | 3% milk | Santa Cruz Biotechnology | |
PPARα | Rabbit | 1:1000 | 3% milk | Abcam | |
HIF-1α | Rabbit | 1:500 | 3% milk | Santa Cruz Biotechnology | |
HPH-2 | Mouse | 1:1000 | 3% milk | Thermo Scientific | |
BNIP3 | Rabbit | 1:2000 | 3% milk | Cell Signaling | |
P53 | Mouse | 1:1000 | 3% milk | Cell Signaling | |
Bax | Rabbit | 1:2000 | 3% milk | Cell Signaling | |
iNOS | Rabbit | 1:1000 | 3% milk | Abcam | |
3-nitrotyrosine | Mouse | 1:1000 | 3% milk | Abcam | |
GAPDH | Rabbit | 1:2000 | 3% milk | Cell Signaling | |
β-actin | Rabbit | 1:2000 | 3% milk | Cell Signaling | |
Lamin A/C | Rabbit | 1:1000 | 3% milk | Cell Signaling |
TTBS represents:Tris buffered saline, pH 7.4 with 0.1% Tween 20.