Figure 3.

Antagonistic action of ethylene and cytokinins on the Pep-triggered acceleration of dark-induced senescence. (A) Leaf discs of Col-0 as well as ein3 eil1 were incubated for 4 days in darkness floating on a solution with 100 nM AtPep1or without any peptide (control). (B) Leaf discs of Col-0 plants were treated with AtPep1 (100 nM) solution or without any peptide (control), and a second set of discs was co-treated with 50 nM trans-Zeatin. Pictures show the discs after 4 days incubation in darkness. Bars represent the mean of total chlorophyll extracted from four leaf discs. Error bars indicate ±1 SE of the mean and asterisks represent Student's t-test results (^*** = p < 0.001).