Abstract
The nicotinamide analogue 6-aminonicotinamide and dibutyryl 3':5'-cAMP inhibit myogenesis in a clonal rat cell line from skeletal muscle. Both reagents produce a similar morphological response in the cells, and stimulate collagen and glycosaminoglycan synthesis. These data suggest that 6-aminonicotinamide and dibutyryl cAMP induce a phenotypic transformation of myogenic cells to cells that share many characteristics with chondrocytes.
Full text
PDF




Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Caplan A. I. Effects of a nicotinamide-sensitive teratogen 6-aminonicotinamide on chick limb cells in culture. Exp Cell Res. 1972 Jan;70(1):185–195. doi: 10.1016/0014-4827(72)90196-6. [DOI] [PubMed] [Google Scholar]
- Caplan A. I. The site and sequence of action of 6-aminonicotinamide in causing bone malformations of embryonic chick limb and its relationship to normal development. Dev Biol. 1972 May;28(1):71–83. doi: 10.1016/0012-1606(72)90127-3. [DOI] [PubMed] [Google Scholar]
- Caplan A. I. The teratogenic action of the nicotinamide analgos 3-acetylpyridine and 6-aminonicotinamide on developing chick embryos. J Exp Zool. 1971 Nov;178(3):351–357. doi: 10.1002/jez.1401780310. [DOI] [PubMed] [Google Scholar]
- DIETRICH L. S., FRIEDLAND I. M., KAPLAN L. A. Pyridine nucleotide metabolism: mechanism of action of the niacin antagonist, 6-aminonicotinamide. J Biol Chem. 1958 Oct;233(4):964–968. [PubMed] [Google Scholar]
- Everse J., Kaplan N. O., Schichor S. Accumulation of oxidized diphosphopyridine nucleotide in mouse liver after administration of reduced diphosphopyridine nucleotide. Arch Biochem Biophys. 1970 Jan;136(1):106–111. doi: 10.1016/0003-9861(70)90332-2. [DOI] [PubMed] [Google Scholar]
- Goggins J. F., Johnson G. S., Pastan I. The effect of dibutyryl cyclic adenosine monophosphate on synthesis of sulfated acid mucopolysaccharides by transformed fibroblasts. J Biol Chem. 1972 Sep 25;247(18):5759–5764. [PubMed] [Google Scholar]
- Heersche J. N., Fedak S. A., Aurbach G. D. The mode of action of dibutyryl adenosine 3',5'-monophosphate on bone tissue in vitro. J Biol Chem. 1971 Nov 25;246(22):6770–6775. [PubMed] [Google Scholar]
- Hsie A. W., Jones C., Puck T. T. Further changes in differentiation state accompanying the conversion of Chinese hamster cells of fibroblastic form by dibutyryl adenosine cyclic 3':5'-monophosphate and hormones. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1648–1652. doi: 10.1073/pnas.68.7.1648. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaukel E., Hilz H. Permeation of dibutyryl cAMP into HeLa cells and its convesion to monobutyryl cAMP. Biochem Biophys Res Commun. 1972 Jan 31;46(2):1011–1018. doi: 10.1016/s0006-291x(72)80242-0. [DOI] [PubMed] [Google Scholar]
- Kidokoro Y. Developmental changes of membrane electrical properties in a rat skeletal muscle cell line. J Physiol. 1975 Jan;244(1):129–143. doi: 10.1113/jphysiol.1975.sp010787. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kidokoro Y., Heinemann S. Synapse formation between clonal muscle cells and rat spinal cord explants. Nature. 1974 Dec 13;252(5484):593–594. doi: 10.1038/252593a0. [DOI] [PubMed] [Google Scholar]
- Kosher R. A., Lash J. W., Minor R. R. Environmental enhancement of in vitro chondrogenesis. IV. Stimulation of somite chondrogenesis by exogenous chondromucoprotein. Dev Biol. 1973 Dec;35(2):210–220. doi: 10.1016/0012-1606(73)90018-3. [DOI] [PubMed] [Google Scholar]
- LANDAUER W., CLARK E. M. The interaction in teratogenic activity of the two niacin analogs 3-acetylpyridine and 6-aminonicotinamide. J Exp Zool. 1962 Dec;151:253–258. doi: 10.1002/jez.1401510306. [DOI] [PubMed] [Google Scholar]
- Landauer W., Sopher D. Succinate, glycerophosphate and ascorbate as sources of cellular energy and as antiteratogens. J Embryol Exp Morphol. 1970 Aug;24(1):187–202. [PubMed] [Google Scholar]
- Mayne R., Sanger J. W., Holtzer H. Inhibition of mucopolysaccharide synthesis by 5-bromodeoxyuridine in cultures of chick amnion cells. Dev Biol. 1971 Aug;25(4):547–567. doi: 10.1016/0012-1606(71)90005-4. [DOI] [PubMed] [Google Scholar]
- McMahon D. Chemical messengers in development: a hypothesis. Science. 1974 Sep 20;185(4156):1012–1021. doi: 10.1126/science.185.4156.1012. [DOI] [PubMed] [Google Scholar]
- Medoff J. Enzymatic events during cartilage differentiation in the chick embryonic limb bud. Dev Biol. 1967 Aug;16(2):118–143. doi: 10.1016/0012-1606(67)90020-6. [DOI] [PubMed] [Google Scholar]
- Nevo Z., Horwitz A. L., Dorfmann A. Synthesis of chondromucoprotein by chondrocytes in suspension culture. Dev Biol. 1972 May;28(1):219–228. doi: 10.1016/0012-1606(72)90139-x. [DOI] [PubMed] [Google Scholar]
- Patrick J., Heinemann S. F., Lindstrom J., Schubert D., Steinbach J. H. Appearance of acetylcholine receptors during differentiation of a myogenic cell line. Proc Natl Acad Sci U S A. 1972 Oct;69(10):2762–2766. doi: 10.1073/pnas.69.10.2762. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Peterkofsky B., Prather W. B. Increased collagen synthesis in Kirsten sarcoma virus-transformed BALB 3T3 cells grown in the presence of dibutyryl cyclic AMP. Cell. 1974 Nov;3(3):291–299. doi: 10.1016/0092-8674(74)90144-5. [DOI] [PubMed] [Google Scholar]
- Richler C., Yaffe D. The in vitro cultivation and differentiation capacities of myogenic cell lines. Dev Biol. 1970 Sep;23(1):1–22. doi: 10.1016/s0012-1606(70)80004-5. [DOI] [PubMed] [Google Scholar]
- Rosenberg M. J., Caplan A. I. Nicotinamide adenine dinucleotide levels in cells of developing chick limbs: possible control of muscle and cartilage development. Dev Biol. 1974 May;38(1):157–164. doi: 10.1016/0012-1606(74)90266-8. [DOI] [PubMed] [Google Scholar]
- SEARLS R. L. ISOLATION OF MUCOPOLYSACCHARIDE FROM THE PRECARTILAGINOUS EMBRYONIC CHICK LIMB BUD. Proc Soc Exp Biol Med. 1965 Apr;118:1172–1176. doi: 10.3181/00379727-118-30072. [DOI] [PubMed] [Google Scholar]
- Schubert D., Heinemann S., Carlisle W., Tarikas H., Kimes B., Patrick J., Steinbach J. H., Culp W., Brandt B. L. Clonal cell lines from the rat central nervous system. Nature. 1974 May 17;249(454):224–227. doi: 10.1038/249224a0. [DOI] [PubMed] [Google Scholar]
- Schubert D., Tarikas H., Humphreys S., Heinemann S., Patrick J. Protein synthesis and secretion in a myogenic cell line. Dev Biol. 1973 Jul;33(1):18–37. doi: 10.1016/0012-1606(73)90161-9. [DOI] [PubMed] [Google Scholar]
- Searls R. L., Janners M. Y. The stabilization of cartilage properties in the cartilage-forming mesenchyme of the embryonic chick limb. J Exp Zool. 1969 Mar;170(3):365–375. doi: 10.1002/jez.1401700313. [DOI] [PubMed] [Google Scholar]
- Seegmiller R. E., Runner M. N. Normal incorporation rates for precursors of collagen and mucopolysaccharide during expression of micromelia induced by 6-aminonicotinamide. J Embryol Exp Morphol. 1974 Apr;31(2):305–312. [PubMed] [Google Scholar]
- Shainberg A., Yagil G., Yaffe D. Alterations of enzymatic activities during muscle differentiation in vitro. Dev Biol. 1971 May;25(1):1–29. doi: 10.1016/0012-1606(71)90017-0. [DOI] [PubMed] [Google Scholar]
- Shimoyama M., Kawai M., Hoshi Y., Ueda I. Nicotinamide inhibition of 3',5'-cyclic AMP phosphodiesterase in vitro. Biochem Biophys Res Commun. 1972 Nov 15;49(4):1137–1141. doi: 10.1016/0006-291x(72)90331-2. [DOI] [PubMed] [Google Scholar]
- Steinbach J. H., Harris A. J., Patrick J., Schubert D., Heinemann S. Nerve-muscle interaction in vitro. Role of acetylcholine. J Gen Physiol. 1973 Sep;62(3):255–270. doi: 10.1085/jgp.62.3.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tarikas H., Schubert D. Regulation of adenylate kinase and creatine kinase activities in myogenic cells. Proc Natl Acad Sci U S A. 1974 Jun;71(6):2377–2381. doi: 10.1073/pnas.71.6.2377. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wahrmann J. P., Winand R., Luzzati D. Effect of cyclic AMP on growth and morphological differentiation of an established myogenic cell line. Nat New Biol. 1973 Sep 26;245(143):112–113. doi: 10.1038/newbio245112a0. [DOI] [PubMed] [Google Scholar]
- Watson J. The influence of intracellular levels of cyclic nucleotides on cell proliferation and the induction of antibody synthesis. J Exp Med. 1975 Jan 1;141(1):97–111. doi: 10.1084/jem.141.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zalin R. J., Montague W. Changes in adenylate cyclase, cyclic AMP, and protein kinase levels in chick myoblasts, and their relationship to differentiation. Cell. 1974 Jun;2(2):103–108. doi: 10.1016/0092-8674(74)90098-1. [DOI] [PubMed] [Google Scholar]
- Zalin R. J. The relationship of the level of cyclic amp to differentiation in primary cultures of chick muscle cells. Exp Cell Res. 1973 Mar 30;78(1):152–158. doi: 10.1016/0014-4827(73)90049-9. [DOI] [PubMed] [Google Scholar]
