Figure 3.

Inflammatory cell infiltration and nuclear factor-κB (NF-κB) activation was evident in homozygous PACE-tryp^on mice. (A) Immunohistochemical staining with a pan-leucocyte marker CD45 revealed that PACE (paired basic amino acid-cleaving enzyme)–trypsinogen led to dramatically increased inflammatory cell infiltration of the pancreas over time (upper panel). Neutrophil infiltration, as indicated by Gr-1 staining, was abundant in the early phase and declined during progression of the disease (middle panel). Infiltration of mature macrophages (surface marker F4/80) occurred relatively late (lower panel) (all panels 100×). (B) NF-κB activation was detected by monitoring p65 nuclear translocation (brown staining, 400×, inserts are higher magnification of nuclei). PACE-tryp^on animals were sacrificed at 3 and 7 days after tamoxifen induction and localisation of p65 was conducted using an anti-p65 antibody.