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. Author manuscript; available in PMC: 2015 Jul 22.
Published in final edited form as: Nat Commun. 2015 Jan 22;6:5779. doi: 10.1038/ncomms6779

Figure 5. F4/80hi macrophages are a main source of CXCL1 and CXCL2 early after Candida infection.

Figure 5

(a) CXCL1 and CXCL2 production by various splenic cell populations. Indicated splenic cell populations were purified by FACS from the spleen of mice 24 hrs after 106 Candida i.v. inoculation, and each population was cultured for 24 hrs (106 cells/ml). The chemokine production in the supernatants was assessed by ELISA. n=3. (b) Splenocytes were obtained from mice 24 hrs after i.v. injection of 106 Candida spores. F4/80hi macrophage population was depleted by magnetic beads, and the rest of splenocytes were confirmed to be lacking the F4/80hi population by flow cytometry (left panels). Whole splenocytes and F4/80hi macrophages-removed splenocytes (F4/80−) were cultured (5×107/ml) for 24 hrs and chemokine production in the supernatants was evaluated by ELISA. n=3. Error bars represent mean ± SD. Student’s t-test was used for statistical analysis. **; p <0.01.