Figure 7. CD66b⁺ neutrophils express less JAM-A and ALCAM than CD14⁺CD16⁺ monocytes.

PBMCs from HIV-negative and -positive individuals (from MHBB and WIHS) were isolated; stained for CD66b, CD14, CD16, JAM-A, and ALCAM; and analyzed by flow cytometry. After subtracting the contribution of the isotype-matched negative control antibodies, the mean fluorescence intensities of (A) JAM-A and (B) ALCAM were determined on CD14⁺CD16⁺ monocytes (open shapes) and CD66b⁺ neutrophils (solid shapes) from HIV-infected individuals in the MHBB (squares) and WIHS (circles) cohorts. (C) JAM-A and (D) ALCAM expression on CD66b⁺ neutrophils was determined on cells isolated from HIV-positive individuals in the MHBB (squares) and WIHS (triangles) cohorts and HIV-negative individuals (circles). Data are represented as mean ± sem. Significance was determined by a two-tailed paired t-test; **P < 0.01, ***P < 0.001.