PBMCs from 4–16 HIV-infected people were added to our in vitro model of the human BBB and allowed to transmigrate for 24 h in response to 200 ng/ml CCL2 or 100 ng/ml CXCL12. After transmigration, the cells were collected, stained for CD3, and quantified by flow cytometry. The percent of CD3+ T cells that transmigrated across the BBB in response to (A) CCL2 or (B) CXCL12 (solid bars) or media alone (open bars) was determined. Chemokine-mediated transmigration in response to (A) CCL2 or (B) CXCL12 was performed in the presence and absence of blocking antibodies to JAM-A (vertical hatching), ALCAM (checkered hatching), or isotype-matched negative controls (horizontal hatching). Data are represented as mean ± sem. Significance was determined by two-tailed paired t-test. *P < 0.05, **P < 0.01.