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. 2014 Nov 11;97(2):285–296. doi: 10.1189/jlb.2A1013-568RRR

Figure 4. Pyk2-deficient cells have normal proximal TCR-induced signaling.

Figure 4.

Control and Pyk2-deficient HuT78 T cells were stimulated with anti-CD3 for various times. (A) Global changes in tyrosine phosphorylation were assessed by antiphosphotyrosine (pY) immunoblotting. Data are representative of 3 independent experiments. pY, . (B and C) The expression of Pyk2 and the site-specific phosphorylation of LAT Y226 (p-LAT) and SLP-76 Y128 (p-SLP-76) were examined by immunoblotting. Data are representative of 3 independent experiments. (C)_The change in the phosphorylation of each of these proteins relative to the maximum Luc sample was calculated. The mean expression of Pyk2 across all experimental time-points was also calculated. In the graphs, dots represent values calculated from an individual stimulation, and the mean of 3 experiments ± sem is shown in the bar graphs and error bars. *P < 0.05 relative to the Luc miRNA control at that time-point.