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. 2014 Nov 24;97(2):379–390. doi: 10.1189/jlb.4A0714-339R

Figure 1. The presence of lp36 is required for maximal IDO production in human PBMCs.

Figure 1.

Human PBMCs were coincubated for 12 h at 37°C with B. burgdorferi strains B31-A3, A3-M9 lp36−, and A3-M9 lp36−/lp36+ at a MOI of 10. PBMC lysates were resolved by 12.5% SDS-PAGE for Western immunoblotting with rabbit anti-human IDO1. Densitometric values of protein expression were normalized to GAPDH and quantitated by use of ImageJ software. Columns show the mean ± sd of PBMCs from 3 donors, assessed in triplicate in independent experiments. Representative Western blot images from 1 donor are shown. One-way ANOVA with Tukey’s post-test was used for statistics. ***P < 0.001; *P < 0.05, relative to PBMCs incubated with medium alone. NS, not significantly different.