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Human PBMCs were coincubated for 12 h at 37°C with B. burgdorferi strains B31-A3, A3-M9 lp36−, and A3-M9 lp36−/lp36+ at a MOI of 10. Cells were immunostained to assess intracellular IDO expression by CD83+CCR7+ mDCs (A) and pDCs (B). Dot plots are representative of 3 donors assessed in triplicate in independent experiments. Five hundred thousand events/sample were collected. FSC, Forward-scatter.
