Table 2. Twelve Genes Randomly Selected for Deep Amplicon Sequencing and Analysisa.
reads with T7 insertion | reads total | insertion frequency | |
---|---|---|---|
acrD | 549 | 452814 | 0.1212% |
edd | 59 | 157788 | 0.0374% |
osmC | 13 | 85100 | 0.0153% |
fryB | 26 | 299019 | 0.0087% |
SodB | 25 | 450003 | 0.0056% |
pssA | 6 | 151518 | 0.0040% |
secE | 15 | 561640 | 0.0027% |
thrL | 4 | 197901 | 0.0020% |
GlnD | 4 | 263023 | 0.0015% |
acrA | 7 | 481259 | 0.0015% |
mdaB | 2 | 237727 | 0.0008% |
hemC | 1 | 129353 | 0.0008% |
The number of reads with a T7 promoter insertion was compared to reads without an insertion to calculate the insertion frequency.