Table 2. Twelve Genes Randomly Selected for Deep Amplicon Sequencing and Analysis^a.

	reads with T7 insertion	reads total	insertion frequency
acrD	549	452814	0.1212%
edd	59	157788	0.0374%
osmC	13	85100	0.0153%
fryB	26	299019	0.0087%
SodB	25	450003	0.0056%
pssA	6	151518	0.0040%
secE	15	561640	0.0027%
thrL	4	197901	0.0020%
GlnD	4	263023	0.0015%
acrA	7	481259	0.0015%
mdaB	2	237727	0.0008%
hemC	1	129353	0.0008%

^aThe number of reads with a T7 promoter insertion was compared to reads without an insertion to calculate the insertion frequency.