Table 3. Primers to Generate pET-nrdA Mutants.
| primer | function | forward primer nucleotide sequence 5′–3′ |
|---|---|---|
| 1 | TAT730 → TTT730 | G GTC AAA ACA CTG TTT TAG CAG AAC ACC CG |
| 2 | TGC439 → GCC439 | GCT CAG TCT AAC CTG GCC CTG GAG ATA GCC C |
| 3 | TGC439 → AGC439 | GCT CAG TCT AAC CTG AGC CTG GAG ATA GCC C |