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. 2014 Dec 25;137(1):10–13. doi: 10.1021/ja5084249

Figure 4.

Figure 4

(a) ARE induction by Keap1-specific HNEylation phenocopies whole-cell HNEylation. Normalized luciferase activity derived from ratio of ARE-inducible firefly to constitutive Renilla luciferase in HEK-293 cells alongside myc-Nrf2 and either Halo-Keap1 or GFP-Keap1 (nontargetable control). (b) mRNA expression of ARE-driven genes induced subsequent to T-REX HNEylation in HEK-293 cells analyzed by qRT-PCR. (c) The expression level of NQO1 assessed by western blot. Also see Figure S9. Error bars in panels a and b designate SD with n = 3 and ≥9, respectively).