Table 1. Product Analysis of Reactions of the Sacculus with the Given Enzymesa,b.
| sacculus/PBP4 | sacculus/PBP4-sol/MltAc | sacculus/PBP4-Insol/MltAc | sacculus/PBP4/MltAd | sacculus/MltA/PBP4e | sacculus/MltA | induced sacculus/MltAf | |
|---|---|---|---|---|---|---|---|
| cross-link/non-cross-linked | |||||||
| noncross-linked | 100 | 87 ± 8 | 68 ± 10 | 70 ± 10 | 97 ± 1 | 53 ± 2 | 50 ± 6 |
| 3,3 cross-link | NDg | 1.5 ± 0.2 | 5 ± 1 | 5 ± 0.3 | 3 ± 1 | 4 ± 1 | 1.4 ± 0.3 |
| 4,3-cross-link | NDg | 11 ± 7 | 26 ± 9 | 25 ± 10 | NDg | 43 ± 3 | 49 ± 5 |
| peptideh | |||||||
| penta | NDg | NDg | 0.06 ± 0.02 | 0.06 ± 0.02 | NDg | 0.05 ± 0.01 | 1.7 ± 0.4 |
| tetra | 89 ± 1 | 81 ± 1 | 64 ± 1 | 66 ± 1 | 79 ± 2 | 75 ± 4 | 82 ± 5 |
| tri | 10 ± 1 | 11 ± 1 | 22 ± 1 | 21 ± 0.2 | 13 ± 3 | 18 ± 3 | 10 ± 3 |
| othersi | 1.2 ± 0.3 | 8 ± 1 | 13 ± 1 | 13 ± 2 | 7 ± 1 | 8 ± 1 | 6 ± 2 |
a
Amounts are expressed as a percentage of the total EIC peak area.
b
Average values of two runs with errors.
c
Sacculus was digested with PBP4, and the soluble and insoluble portions were separated by centrifugation. Each portion was reacted separately with MltA.
d
Combined results of the soluble and insoluble portions.
e
Sacculus was reacted with MltA, followed by reaction with PBP4.
f
Sacculus was isolated from bacteria grown in the presence of sub-MIC concentrations of cefoxitin and reacted with MltA.
g
ND, not detected.
h
Cross-linked and non-cross-linked muropeptide containing the corresponding peptide.
i
Less abundant peptides, TetraGly, TriGly, TriLys, Di, and no peptide.