. 2014 Dec 11;137(1):190–200. doi: 10.1021/ja5111706

Table 2. Steady-State Kinetic Parameters for Turnover of the Synthetic Substrates by PBP4^a.

	k_cat (s^–1)	K_m (mM)	k_cat/K_m (M^–1 s^–1)
18	24.0 ± 1.9	3.1 ± 0.5	7700 ± 1400
19	14.1 ± 1.5	1.3 ± 0.3	11000 ± 2800
20	47.0 ± 6.9	2.4 ± 0.5	20000 ± 5100

Reactions were carried out in 50 mM Tris buffer, 100 mM NaCl, pH 7.6 at 25 °C, and they were quenched after 30 min. At pH 8.5, 50 mM Tris buffer, 100 mM NaCl, rate values for substrates were higher by 1.3-fold.

Table 2. Steady-State Kinetic Parameters for Turnover of the Synthetic Substrates by PBP4a.

Table 2. Steady-State Kinetic Parameters for Turnover of the Synthetic Substrates by PBP4^a.