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. 2014 Dec 27;6(1):130–137. doi: 10.1021/cn500288n

Figure 3.

Figure 3

U0126 reduces H2O2-induced ROS level in PC12 cells. (a) Representative images of the green channel (DCHFDA fluorescence) at 10 min and 2 h after H2O2 addition. The fluorescence intensity is correlated with the amount of ROS in cells. All images are shown with the same intensity scale bar. (b) Quantitative measurements of the fluorescence intensity show that U0126 and U0124 are able to reduce the amount of ROS in PC-12 cells within 10 min of incubation. The ROS level is maintained low at 2 h. Trametinib increases the amount of ROS in PC-12 cells. Images are collated from three sets of individual experiments and the error bars depict ± SD. Scale bar = 100 μm.