Fig. 5.

Secretion of TNF-α, IL-1β, and IL-6 in serum from LPS-injected Balb/c. Secretory protein levels of TNF-α, IL-1β, and IL-6 from the serum were measured by the ELISA method for the indicated times. All results were representative of three separate experiments. (A) injection of LPS resulted in a marked increase of TNF-α which was observed as early as at 2 h and reached a primary peak level with 1,591.15±5.798 pg/ml at 2 h, and thereafter gradually decreased in a time-dependent manner. (B) LPS also increased in secretion of IL-1β, which was already reached a peak level with 183.2604±6.542 pg/ml at 4 h, thereafter sustained its level up to 12 h, and gradually decreased in a time-dependent manner. (C) IL-6 secretion was already reached a peak level with 766.3889±8.214 pg/ml at 2 h, and thereafter gradually decreased in a time-dependent manner.