Table 1.
Details of primary and secondary antibodies used for IHC
| Primary Abs (Company details) | Primary Ab dilutions | Secondary Abs (Company details) | Secondary Ab dilutions |
|---|---|---|---|
| Ki-67 (Dako Cytomation, Denmark) | 1 : 50 | Alexa Fluor 488 anti-mouse (Invitrogen, Paisley, UK) | 1 : 200 |
| Barboni et al. (2012a,b) | |||
| Cx43 (Chemicon Int. Billrerica, MA, USA) | 1 : 200 | Alexa Fluor 488 anti-mouse (Invitrogen, Paisley, UK) | 1 : 500 |
| Barboni et al. (2012a) | |||
| Cx32 (Chemicon Int. Billrerica, MA, USA) | 1 : 200 | Alexa Fluor 488 anti-mouse (Invitrogen, Paisley, UK) | 1 : 750 |
| Barboni et al. (2012a) | |||
| COL1 (Chemicon Int. Billrerica, MA, USA) | 1 : 100 | Alexa Fluor 488 anti-mouse (Invitrogen, Paisley, UK) | 1 : 200 |
| Barboni et al. (2012a,b) | |||
| COL3 (Chemicon Int. Billrerica, MA, USA) | 1 : 500 | Alexa Fluor 488 anti-mouse (Invitrogen, Paisley, UK) | 1 : 200 |
| Barboni et al. (2012b) | |||
| TGF-β1 (Abcam, Cambridge, UK) | 1 : 100 | Alexa Fluor 488 anti-mouse (Invitrogen, Paisley, UK) | 1 : 200 |
| Barboni et al. (2012b) | |||
| VWF (Dako Cytomation, Denmark) | 1 : 400 | Alexa Fluor 488 anti-rabbit (Invitrogen, Paisley, UK) | 1 : 200 |
| Barboni et al. (2012b, 2013) | |||
| VEGF (Novus Biologicals, Littleton, CO, USA) | 1 : 10 | Alexa Fluor 488 anti-rabbit (Invitrogen, Paisley, UK) | 1 : 200 |
| Barboni et al. (2012b, 2013) | |||
| NF200 (Sigma-Aldrich, St. Louis, MO, USA) | 1 : 500 | Alexa Fluor 488 anti-rabbit (Invitrogen, Paisley, UK) | 1 : 250 |
| Barboni et al. (2014) | |||
| NGF (Sigma-Aldrich, St. Louis, MO, USA) | 1 : 400 | Alexa Fluor 488 anti-rabbit (Invitrogen, Paisley, UK) | 1 : 250 |
| Barboni et al. (2002), Mattioli et al. (1999) | |||
| OCN (Abcam, Cambridge, UK) | 1 : 50 | Alexa Fluor 488 anti-mouse (Invitrogen, Paisley, UK) | 1 : 400 |
| Barboni et al. (2012a, 2013) | |||
| TERT (Calbiochem, Gibbstown, NJ, USA) | 1 : 250 | Alexa Fluor 568 anti-rabbit (Invitrogen, Paisley, UK) | 1 : 250 |
| Russo et al. (2007), Mauro et al. (2010), Barboni et al. (2012a) | |||
| SOX2 (Abcam, Cambridge, UK) | 1 : 200 | Alexa Fluor 488 anti-rabbit (Invitrogen, Paisley, UK) | 1 : 200 |
| Barboni et al. (2012a) | |||
| NANOG (Millipore, Billerica, MA, USA) | 1 : 500 | Alexa Fluor 488 anti-rabbit (Invitrogen, Paisley, UK) | 1 : 200 |
| Barboni et al. (2012a, 2014) |
Primary and secondary antibodies (Abs) were diluted in PBS supplemented with 1% BSA. Cx, connexin; NGF, nerve growth factor; NF200, neurofilament 200; TGF, transforming growth factor; VEGF, vascular endothelial growth factor.