Table 2.
Primer sequences used for RT-PCR
| Gene | Accession number | Primer sequence | Product size (bp) | PCR cycles |
|---|---|---|---|---|
| GADPH | AF030943.1 | F: CCTGCACCACCAACTGCTTG | 224 | 40 |
| Ovine | R: TTGAGCTCAGGGATGACCTTG | |||
| COL1 | AF129287.1 | F: CGTGATCTGCGACGAACTTAA | 212 | 40 |
| Ovine | R: GTCCAGGAAGTCCAGGTTGT | |||
| COL3 | AY091605.1 | F: AAGGGCAGGGAACAACTTGAT | 355 | 40 |
| Ovine | R: GTGGGCAAACTGCACAACATT | |||
| TNMD | NM_001099948.1 | F: TGGTGAAGACCTTCACTTTCC | 352 | 40 |
| Bos Taurus | R: TTAAACCCTCCCCAGCATGC | |||
| THBS4 | NM_001034728.1 | F: CCGCAGGTCTTTGACCTTCT | 231 | 40 |
| Bos Taurus | R: CAGGTAACGGAGGATGGCTTT | |||
| SCXB | XM_866422.2 | F: AACAGCGTGAACACGGCTTTC | 299 | 45 |
| Bos Taurus | R: TTTCTCTGGTTGCTGAGGCAG | |||
| OCN | DQ418490.1 | F: AGACACCATGAGAACCCCCAT | 234 | 40 |
| Ovine | R: TTGAGCTCACACACCTCCCT | |||
| NANOG | FJ970651.1 | F: TGGATCTGCTTATTCAGGACAG | 209 | 40 |
| Ovine | R: TGCTGGAGGCTGAGGTATTTC | |||
| SOX2 | X96997.1 | F: ACCAGAAGAACAGCCCGGAC | 264 | 45 |
| Ovine | R: TCATGAGCGTCTTGGTTTTCCG | |||
| TERT | EU139125.1 | F: TTGTCCCCGCAGGTGTCTTG | 176 | 45 |
| Ovine | R: TGACCGTGTTGGGCAGGTAG | |||
| OCT4A | NM_174580.1 | F: TATGACTTGTGTGGAGGGATG | 327 | 45 |
| Bos Taurus | R: AAACAGAACCCCCAGGGTGA |
PCR, polymerase chain reaction.