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. 2015 Jan 17;9:575–601. doi: 10.2147/DDDT.S75221

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© 2015 Wang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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ALS downregulates the expression of CDK1/CDC2, CDK2, cyclin B1, and cyclin D1 but upreregulates the expression of p21 Waf1/Cip1, p27 Kip1, and p53 in PANC-1 and BxPC-3 cells.

Notes: Cells were treated with ALS at 0.1 μM, 1 μM, and 5 μM for 24 hours and then the protein samples were subject to Western blotting assay. (A) Representative blots for the expression of CDK1/CDC2, CDK2, cyclin B1, cyclin D1, p21 Waf1/Cip1, p27 Kip1, and p53 in PANC-1 and BxPC-3 cells. (B) Bar graphs showing the relative expression levels of CDK1/CDC2, CDK2, cyclin B1, cyclin D1, p21 Waf1/Cip1, p27 Kip1, and p53 in PANC-1 and BxPC-3 cells. Data represent the mean ± SD of three independent experiments. *P<0.05, **P<0.01, and ***P<0.001 by one-way ANOVA.

Abbreviations: ALS, alisertib; CDK, cyclin-dependent kinase; SD, standard deviation; ANOVA, analysis of variance.

ALS downregulates the expression of CDK1/CDC2, CDK2, cyclin B1, and cyclin D1 but upreregulates the expression of p21 Waf1/Cip1, p27 Kip1, and p53 in PANC-1 and BxPC-3 cells.

Notes: Cells were treated with ALS at 0.1 μM, 1 μM, and 5 μM for 24 hours and then the protein samples were subject to Western blotting assay. (A) Representative blots for the expression of CDK1/CDC2, CDK2, cyclin B1, cyclin D1, p21 Waf1/Cip1, p27 Kip1, and p53 in PANC-1 and BxPC-3 cells. (B) Bar graphs showing the relative expression levels of CDK1/CDC2, CDK2, cyclin B1, cyclin D1, p21 Waf1/Cip1, p27 Kip1, and p53 in PANC-1 and BxPC-3 cells. Data represent the mean ± SD of three independent experiments. *P<0.05, **P<0.01, and ***P<0.001 by one-way ANOVA.

Abbreviations: ALS, alisertib; CDK, cyclin-dependent kinase; SD, standard deviation; ANOVA, analysis of variance.