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. Author manuscript; available in PMC: 2016 Feb 20.
Published in final edited form as: Mol Biosyst. 2014 Nov 24;11(2):338–353. doi: 10.1039/c4mb00627e

Figure 7. Representative protease enzymes used in in vitro peptide synthesis.

Figure 7

(A) Convergent (4 + 4) synthesis of a highly functionalized octapeptide with an overall yield of ~ 40% and utilization of seven different proteases. (B) Convergent approach towards the synthesis of the human thyroid PKA (protein kinase A)-anchoring protein Ht31. The carboxyl component Boc-Asp-Leu-Ile-Glu-Glu-Ala-Ala-Ser-OGp was synthesized by oxime-resin strategy, and the hexadecapeptide fragment synthesized by standard peptide chemistry. The final ligation step utilized chymotrypsin in vitro, to afford the protected oligopeptide which could be deprotected in the final step to obtain a fully functionalized Ht31(final yield ~ 30%).