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. 2014 Dec 30;13:179. doi: 10.1186/s12934-014-0179-z

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© Fleetwood et al.; licensee BioMed Central. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Different inducible promoters compared to the constitutive aidA promoter for surface expression of recombinant protein. A. Representative dot plot from flow-cytometric analysis of E. coli BL21 (paidA-Z_IgG-EC). Fluorescence intensity corresponding to IgG-binding on the y-axis and fluorescence intensity corresponding to surface expression level (HSA-binding) on the x-axis. B. The different inducible promoters AraBAD, RhaBAD and T7 before the expression cassette (EC). C. Representative dot plot from flow-cytometric analysis of E. coli BL21 (pRhaBAD-Z_IgG-EC). D. Representative dot plot from flow-cytometric analysis of E. coli BL21 (pAraBAD-Z_IgG-EC).