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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Exp Cell Res. 2014 Dec 9;331(1):1–10. doi: 10.1016/j.yexcr.2014.11.016

Figure 4. BAD regulates EMT-related proteins.

Figure 4

(A) Expression of MTA3, SNAIL, and CXCR4 were assayed by WB in control and conditionally BAD induced MCF7 cells (n=3). Expression of actin and GAPDH are shown as protein loading controls. (B) Relative SNAIL mRNA expression was determined by qPCR in 3 individual experiments with comparable results; representative results from one experiment are shown. (C) Flow cytometric analysis of CXCR4 in control and conditionally BAD-induced MCF7 cells. MCF7 cells containing the tetracycline regulator, but not the BAD insert, were used as a control to show lack of effect of tetracycline by itself. Experiment was repeated 4 times with comparable results. Values represent the mean ± S.E. **p<0.01, ***p<0.001 by Student’s t-test. (D) Expression of Sp1 was assayed by WB in control and conditionally BAD induced MCF7 cells (n=3). Actin was used as loading control.