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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Exp Cell Res. 2014 Dec 9;331(1):1–10. doi: 10.1016/j.yexcr.2014.11.016

Figure 5. BAD regulates ERK and AKT pathways.

Figure 5

(A) MCF7 cells were transiently transfected with indicated plasmid vectors and were growth arrested with 10nM ICI and 0.1% serum for 48 h prior to stimulation with 5% serum or 10 nM E2. Whole cell lysate (50 μg) of each sample was resolved using SDS-PAGE and immunoblotted with indicated antibodies. (B) After BAD induction for 72 hrs, cells were serum-starved for 24 hrs and stimulated with serum for 1 hr (experiments in duplicate). Lysates from BAD induced and control cells were probed with p-ERK and ERK antibodies. (C) Phospho and total AKT were measured by ELISA and phospho/total (p/T) ratios are shown. Values represent the mean ± S.E. **p<0.01, ***p<0.001 (n=3). (D) Induction of AKT after silencing BAD by siRNA.