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In order to determine the structure of the trimeric complex ABC, several samples need to be measured: (a) the monomers A, B and C, (b) the dimeric complexes AB and BC and (c) the trimeric complex (assembly) ABC; (d) the complex ABC is then measured in different buffer conditions containing the ligands/additives required for activity and/or nonhydrolysable substrate analogues to isolate the various stages of the reaction.
