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. 2015 Jan 23;11(1):e1004620. doi: 10.1371/journal.ppat.1004620

Figure 1. IGR1 and IGR2 function as promoters in Arabidopsis cells.

Figure 1

(A) Arrangement of the coding sequences of the IaaH, IaaM and Ipt oncogenes and the intergenic regions (IGRs) in the T-DNA region of the Ti plasmid of A. tumefaciens strain C58, pTiC58. (B) Arabidopsis crown gall callus cells expressing the green fluorescing protein (GFP) under the control of IGR1a (IGR1a::GFP), IGR1b (IGR1b::GFP) and IGR2 (IGR2::GFP). IGR1 was used in two orientations; one is upstream of IaaH CDS (IGR1a) and the other upstream of IaaM (IGR1b). The universal cauliflower mosaic virus promoter was used as a positive control (2× CaMV35S::GFP) and the GFP CDS without promoter, as the negative control (GFP). Images show crown gall callus cells in the transmission microscopy (top row) and the UV light mode (bottom row, excitation: 490 nm, emission: 510 nm). The UV-light intensity used for excitation is the same for both pictures. Bars, 50 μm.