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. 2015 Jan 23;11(1):e1004620. doi: 10.1371/journal.ppat.1004620

Figure 6. WRKY40 binds to the Ipt promoter in vitro.

Figure 6

(A) Positions of W-boxes (TGAC, grey bars) in the sense (above the line) and anti-sense strand (below the line) of the Ipt promoter. The line below the Ipt promoter (−184 bp to −135 bp) indicates the fragment used as Ipt probe for electrophoretic mobility shift assay (EMSA). (B) EMSA with the labeled Ipt promoter probe in the absence (−) and in the presence of 150 ng (+) or 300 ng (++) of purified recombinant histidine-tagged WRKY40 protein. Competitor indicates without (−) and with (+) addition of unlabeled Ipt promoter probe. The WRKY40-Ipt complex indicates binding of WRKY40 protein to the labeled Ipt probe and the free Ipt probe no protein binding.