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a) NaCl dependency of DCL activities. S23 extract was used to calculate optimal NaCl concentration required for DCL activities by providing labelled substrates. The extracts were first desalted by passing two times through sephadex G25 columns with buffers having 5 mM of the salt. Range of concentrations of NaCl used is shown. b) KAc dependency of DCL activities. Further details are similar to a.
