Figure 5.

Chronic Lipid-Rich Diet Does Not Affect Lifespan and Glucose Level in Upd3 Null Drosophila

(A) Survival of upd3 null on control and lard diets. Log-rank, χ² = 0.2264, p = 0.6342; Wilcoxon, χ² = 0.1449, p = 0.7035. Data are pooled from eight independent experiments.

(B) Socs36E transcripts in upd3 null flies and w¹¹¹⁸ controls. Three flies per sample; histogram represents mean ± SEM of four or five independent samples per diet and per time point.

(C) Time course of glucose and trehalose concentrations in flies as in (B) on a control or lard diet. Histograms represent mean ± SEM from four or five samples of three flies per diet.

(D) TLC analysis of TG content of upd3 null flies and w¹¹¹⁸ controls fed a control or lard diet for ten days. Histogram represents quantification of TG; n = 4, mean ± SEM.

(E) Left: Survival of inducible plasmatocyte (HmlΔ)-driven overexpression of upd3 (HmlΔGal80^ts> upd3, closed shapes) compared to control flies (UAS-upd3/+, open shapes). Log-rank, χ² = 56.16, p < 0.0001; Wilcoxon, χ² = 46.46, p < 0.0001 for flies fed a control diet. Center: Survival of inducible fat body (c564)-driven overexpression of upd3 (c564Gal80^ts > upd3, closed shapes) compared to controls (UAS-upd3/+, open shapes). Data are pooled from two independent experiments. Log-rank, χ² = 97.38, p < 0.0001; Wilcoxon, χ² = 88.73, p < 0.0001 for flies fed a control diet. Right: Survival of plasmatocyte (HmlΔ)-driven overexpression of upd2 (HmlΔ > upd2, closed shapes) compared to controls (UAS-upd2/+, open shapes). Data are pooled from three independent experiments. Log-rank, χ² = 119.5, p < 0.0001; Wilcoxon, χ² = 102.7, p < 0.0001 for flies fed a control diet. Please also see Figure S5.