Figure 6.

Macrophage Depletion in Lipid-Rich Diet Rescues Drosophila Lifespan and Glucose Metabolism

(A) DiI-LDL (magenta) uptake 1 hr after injection by HmlΔ⁺ plasmatocytes (green). White cells represent the overlay of GFP and DiI-LDL. Bar, 100 μm. Pie chart: percent colocalization of HmlΔ⁺ cells with DiI-LDL, mean ± SD, p < 0.0001, n = 3.

(B) Sum GFP fluorescence intensity in upd3-Gal4,UAS-GFP reporter flies injected with DiI-LDL or PBS. Mean ± SEM, n = 4.

(C) Confocal images of plasmatocyte-depleted and control flies injected with DiI-LDL (see Experimental Procedures). n = 4.

(D) Survival of plasmatocyte-depleted (HmlΔGal80^ts > rpr, closed shapes) and control flies (HmlΔGal80^ts/+, open shapes) fed a control or lard diet. Data are pooled from four independent experiments. Log-rank, χ² = 75.53, p < 0.0001; Wilcoxon, χ² = 68.46, p < 0.0001 for flies fed a lard diet.

(E) upd3 and Socs36E transcripts in flies as in (D) fed lard or control diets for 20 days; three flies per sample; histogram represents mean ± SEM of four or five independent samples per diet.

(F) Total glucose and trehalose content of flies as in (D) on control or lard diet for 30 days. Histograms represent mean ± SEM from four or five samples of three flies per diet.

(G) TLC analysis of triglyceride contents of groups of ten flies as in (D) fed control or lard diets for 20 days. Histogram represents quantification of triglyceride (TG); n = 4, mean ± SEM. Please also see Figure S6.