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. 2014 Dec 17;16(1):44–62. doi: 10.15252/embr.201439583

Figure 3. Differential expression of Hsa21 genes and non-Hsa21 genes in hematopoietic and non-hematopoietic cells.

Figure 3

Human Gene Expression Profiling (GEP) datasets from public repositories were extracted, and differential expression analysis was performed using the limma algorithm (see Supplementary Tables S1 and S2). The data analyzed here are from references 187,189,190. In order to compare gene expression by these non-hematopoietic cells with non-malignant hematopoietic cells, we used our own unpublished gene expression data from human trisomy 21 (n = 4) and normal (n = 3) second-trimester fetal liver CD34+ HSPC samples obtained using the Affymetrix HuEX_1-0_st array. An FDR cutoff of 0.15 was used. There was almost no overlap between the differentially expressed genes in different tissue types: Only a single Hsa21 gene, MCM3AP (minichromosome maintenance complex component 3-associated protein) (A), and no non-Hsa21 genes were differentially expressed in both hematopoietic and non-hematopoietic cells (B).