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. 2014 Nov 28;16(1):97–106. doi: 10.15252/embr.201438976

Figure 1. YME1L is a stress-sensitive mitochondrial protease.

Figure 1

  1. Illustration showing the oligomer assembly and active site orientation of YME1L (red), AFG3L2 (blue), and paraplegin (orange) in the mitochondrial inner membrane.
  2. Representative immunoblot of lysates prepared from SHSY5Y cells treated with the indicated concentration of H2O2 for 6 h.
  3. Quantification of YME1L and AFG3L2 from immunoblots as shown in (B). Error bars show SEM for n ≥ 3. ***P-value < 0.001, **P-value < 0.01, *P-value < 0.05.
  4. Representative immunoblot of lysates prepared from SHSY5Y cells treated with As(III) (50 μM) for the indicated time.
  5. Quantification of YME1L and AFG3L2 in SHSY5Y cells treated with As(III) (50 μM; 9 h) from immunoblots as shown in (D). Error bars show SEM for n = 4. *P-value < 0.05.
  6. Immunoblots of lysates prepared from HEK293T cells cultured in galactose-supplemented media treated with the indicated concentration of antimycin A for 6 h.
  7. Representative immunoblot of lysates prepared from SHSY5Y cells treated as indicated with cycloheximide (CHX; 50 μg ml−1) and H2O2 (100 μM) for the indicated time.
  8. Quantification of YME1L protein levels from immunoblots as shown in (G). Error bars show SEM for n = 3. *P-value < 0.05.

Source data are available online for this figure.