Figure 4. Site-directed mutagenesis of FER kinase domain.

1. FER protein domains and positions of the mutations introduced for complementation assays. The bold S, T, and S indicate targets for mutagenesis in the activation segment.
2. Box plots from complementation assays showing the percentage of unfertilized ovules for control plants and T1 transformants for each of the site-directed mutagenesis constructs. **** indicates lines with % unfertilized ovules significantly different from untransformed controls of the same genotype (P-values < 0.0001 in t-tests). See Supplementary Table S2 for raw seed count data.