Table 1. Macromolecule-production information.
Source organism | S. melliferum KC3 |
DNA source | S. melliferum KC3 |
Forward primer† | GGTGTACATATGTCAAAAAAAGAACTAGC |
Reverse primer† | CTTTCGGAATTCTTAATTATTGTTTAAATCAG |
Cloning vector | pET-21d |
Expression vector | pET-21d modified by an N-terminal His6 tag followed by a TEV protease digestion site |
Expression host | E. coli BL21-CodonPlus (DE3)-RIPL |
Complete amino-acid sequence of the construct produced‡ | MGSDKIHHHHHH ENLYFQGHMSKKELAAQIAEKFTDVLSKTHAEEITNFVFDHIKKALVAGKEVSIAGFGKFAVTERAARDGRNPSTGETIKIPASKSAKFKAGKQLKTDLNNN |
Restriction sites are underlined.
The N-terminal His6 tag is shown in italics and the TEV protease digestion site is underlined.