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. 2015 Jan 1;71(Pt 1):24–27. doi: 10.1107/S2053230X14025333

Table 1. Macromolecule-production information.

Source organism S. melliferum KC3
DNA source S. melliferum KC3
Forward primer GGTGTACATATGTCAAAAAAAGAACTAGC
Reverse primer CTTTCGGAATTCTTAATTATTGTTTAAATCAG
Cloning vector pET-21d
Expression vector pET-21d modified by an N-terminal His6 tag followed by a TEV protease digestion site
Expression host E. coli BL21-CodonPlus (DE3)-RIPL
Complete amino-acid sequence of the construct produced MGSDKIHHHHHH ENLYFQGHMSKKELAAQIAEKFTDVLSKTHAEEITNFVFDHIKKALVAGKEVSIAGFGKFAVTERAARDGRNPSTGETIKIPASKSAKFKAGKQLKTDLNNN

Restriction sites are underlined.

The N-terminal His6 tag is shown in italics and the TEV protease digestion site is underlined.