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| Method | Hanging-drop vapour diffusion |
| Plate type | Linbro (24-well) |
| Temperature (K) | 277 |
| Protein concentration (mgml1) | 14 |
| Buffer composition of protein solution | 40mM Tris pH 8.0, 200mM NaCl, 5% glycerol |
| Composition of reservoir solution | 0.1M Tris pH 8.0, 35%(v/v) PEG 400, 5% glycerol |
| Volume and ratio of drop (protein:precipitant) | 300nl, 1:2 |
| Volume of reservoir (l) | 400 |
