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. 2015 Jan 1;71(Pt 1):41–44. doi: 10.1107/S2053230X14025461

Table 2. Crystallization.

Method Vapour diffusion
Plate type Hanging drop
Temperature (K) 293
Protein concentration (mgml1) 10
Buffer composition of protein solution 20mM TrisHCl pH 7.5
Composition of reservoir solution 0.6M sodium citrate tribasic, 0.1M TrisHCl pH 7.0
Volume and ratio of drop 2l protein:1l precipitant
Volume of reservoir (l) 150