Table 1. Macromolecule-production information.

The NdeI restriction site and the amino acids added to the wild-type ScoMcrA are underlined.

Source organism	S. coelicolor
DNA source	Genomic DNA
Forward primer	CATATGGCACCTTCGGAGAT
Reverse primer	GGGCTCAGGCAGCGTAAT
Cloning vector	pBluescript
Expression vector	pET-28a
Expression host	E. coli BL21(DE3)
Complete amino-acid sequence of the construct produced	MGSSHHHHHHSSGLVRGSHMASEITRAGILQAIAEHDRIGEAFRATYGFHAATSYFLEHEGRLYDSKAIAGVAHMYDFGVALKSSGLSGGLKHAVAWLRREGFTIREAKTFHRRVGDVRARRAMGALHRVLLLWAIGQAVARARLQWSTTRDAVALMEKYGQVEDGVDGVRYFWALVRDDLWCVEQAEELTLTSRGRRTLESLNAVDSAGLREDDYNLLRSQEAAASAAAGLIARYFHLLAGLLEDFGLHELLAGRWDALRLLGETFKDRDAIWRAYGGQKMAGIGCLADGILSAFSDDKGYADGRIDTTWIAYVGDGLSGDQKLTDGNELMAEHQAVGRALRYWHKFQGQWSFETWAVIVQRRLRWGLGEDKLRREFLWVLAVSERETWEVLEALEADTGELHDDTGDYRSDLALTGADGTESDDEAYRRLAQKAEANAERRGQLKKTVADKYVRDSARGAVLKRCQKRCENECAGHTELTKAGLILQVDHVNDLAKGGDVWNMIALCNCHALKTYGANKVRLQRLLAATARRLHEEKLQ