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| Method | Vapour diffusion |
| Plate type | 24-well hanging-drop plate (Hampton Research) |
| Temperature (K) | 287 |
| Protein concentration (mgml1) | 10 |
| Buffer composition of protein solution | 10mM TrisHCl pH 8.0, 100mM NaCl, 2mM DTT |
| Composition of reservoir solution | 0.2M lithium sulfate monohydrate, 0.1M TrisHCl pH 8.5, 13%(w/v) PEG 4000 |
| Volume and ratio of drop (l) | 1.0:1.0 |
| Volume of reservoir (l) | 160 |
