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Size-exclusion chromatography–multi-angle light scattering (SEC-MALS) analysis of the periplasmic domain of PorM. Chromatograms of the purified periplasmic domain of PorM (pPorM; blue) and of the digested pPorM (pPorM-T; red) are shown. The measured molar masses are ∼110 and ∼60 kDa for pPorM and pPorM-T, respectively. The inset shows an SDS–PAGE of the samples used for SEC-MALS analysis.
